Evolution of secondary metabolites in legumes (Fabaceae)

AbstractLegumes produce a high diversity of secondary metabolites which serve as defence compounds against herbivores and microbes, but also as signal compounds to attract pollinating and fruit-dispersing animals. As nitrogen-fixing organisms, legumes produce more nitrogen containing secondary metabolites than other plant families. Compounds with nitrogen include alkaloids and amines (quinolizidine, pyrrolizidine, indolizidine, piperidine, pyridine, pyrrolidine, simple indole, Erythrina, simple isoquinoline, and imidazole alkaloids; polyamines, phenylethylamine, tyramine, and tryptamine derivatives), non-protein amino acids (NPAA), cyanogenic glucosides, and peptides (lectins, trypsin inhibitors, antimicrobial peptides, cyclotides). Secondary metabolites without nitrogen are phenolics (phenylpropanoids, flavonoids, isoflavones, catechins, anthocyanins, tannins, lignans, coumarins and furanocoumarins), polyketides (anthraquinones), and terpenoids (especially triterpenoid, steroidal saponins, tetraterpenes). While some secondary metabolites have a wide distribution (flavonoids, triterpenes, pinitol), however, others occur in a limited number of taxa. The distributions of secondary metabolites with an irregular occurrence are mapped on a molecular phylogeny of the Fabaceae, reconstructed from a combined data set of nucleotide sequences from rbcL, matK and ITS genes. In most cases, the distribution patterns of secondary metabolites do not agree with the phylogeny of the plants producing them. In contrary, the distribution of many secondary metabolites is patchy and irregular. Thus, the use of phytochemical data to reconstruct a phylogeny of plants is often not informative and can be misleading. The patchy distribution may be due to convergent evolution, a contribution of endophytic fungi or more likely, to an early acquisition of the key genes of secondary metabolism in the evolution of land plants among others by horizontal gene transfer from bacteria. Thus it would be a matter of gene regulation whether these genes are active in some but not all taxa

Mode of action and toxicology of plant toxins and poisonous plants

Abstract Plants have evolved the strategy to produce bioactive natural products as a means of defence against herbivores and microbes. Some plants produce toxins that can severely damage or kill a herbivore. The molecular mode of action of neurotoxins, cytotoxins, metabolic poisons, mutagens and toxins that affect skin and mucosal tissues are summarised and discussed. Important poisonous plants of Europe, their toxins and toxicology are tabulated, as this group of plants can provide lead compounds for the development of natural pesticides against insects, slugs or rodents

Effects of a Novel Dental Gel on Plaque and Gingivitis: A Comparative Study.

ObjectivesThe goal of this prospective, randomized, controlled, double-blinded study was to evaluate the effects of a novel dental gel on plaque and gingival health. The dental gel was designed to (1) break up and prevent re-accumulation of microbial biofilm, and (2) inhibit metal mediated inflammation.Materials and methodsTwenty-five subjects with moderate gingival inflammation (Löe and Silness Gingival Index ≥2) and pocket depths <4 were randomly assigned to brush twice daily for 21 days with the test or the control dental gel. On Days 0, 7, 14 and 21, plaque levels (Quigley-Hein, Turesky Modification Plaque Index), gingival inflammation (Löe and Silness Gingival Index) and gingival bleeding (modified Sulcus Bleeding Index) were determined by one blinded, investigator using a pressure sensitive probe.ResultsAfter 3 weeks, all 3 clinical indices were significantly improved in both groups (P<0.05) and significantly lower in the test group (P<0.05).ConclusionThe novel dental gel formulation was provided effective plaque control and reduced gingival inflammation.Clinical relevanceA novel dentifrice formulation may be an effective tool for plaque removal and maintaining gingival health

On the complex structure of Yang-Mills theory

We consider the coupled set of spectral Dyson-Schwinger equations in Yang-Mills theory for ghost and gluon propagators, which gives us access to the ghost and gluon spectral functions. The set-up is used for a systematic analytic evaluation of the constraints on generalised spectral representations in Yang-Mills theory that are most relevant for informed spectral reconstructions. We also provide numerical results for the coupled set of spectral functions for a large range of potential mass gaps of the gluon, and discuss the limitations and extensions of the present work.Comment: 30 pages, 16 figure

On the quark spectral function in QCD

We calculate the spectral function of light quark flavours in 2+1 flavour vacuum QCD in the isospin-symmetric approximation. We employ spectral Dyson-Schwinger equations and compute the non-perturbative quark propagator directly in real-time, using recent spectral reconstruction results from Gaussian process regression of gluon propagator data in 2+1 flavour lattice QCD. Our results feature a pole-like peak structure at time-like momenta larger than the propagator's gapping scale as well as a negative scattering continuum, which we exploit assuming an analytic pole-tail split during the iterative solution. The computation is augmented with a general discussion of the impact of the quark-gluon vertex and the gluon propagator on the analytic structure of the quark propagator. In particular, we investigate under which conditions the quark propagator shows unphysical complex poles. Our results offer a wide range of applications, encompassing the ab-initio calculation of transport as well as resonance properties in QCD.Comment: 17 pages, 7 figure

Het meten van kennisdoorwerking : meten van de doorwerking van onderzoekskennis in de biologische landbouw : onderzoeksproject "dierenwelzijn in de biologische melkveehouderij"

Het project “Kennisbenutting in de Biologische Landbouw” ontwikkelt een methode voor het meten van de doorwerking van onderzoekskennis in de biologische praktijk. Tegelijk met het ontwikkelen wordt de methode toegepast in verschillende pilots. Zo wordt de aanpak ontwikkeld, toegepast en getest. Deze pilot gaat over onderzoek naar dierenwelzijn in de biologische melkveehouderij (koeien). Het betreft hier een groep van projecten die in een periode van ongeveer vier jaar zijn uitgevoerd. Welzijn van dieren heeft uiteenlopende aspecten die vaak in afzonderlijke projecten zijn onderzocht. In de praktijk van het boerenbedrijf moet de ondernemer een keuze maken hoe hij de diverse welzijnsaspecten met elkaar combineert om tot een aanpak voor zijn eigen bedrijf te komen. De complexiteit van deze casus is dan ook de vertaling van een brede projectenportfolio naar meetbare benutting in de bedrijfspraktijk

Average nucleotide identity of genome sequences supports the description of Rhizobium lentis sp. nov., Rhizobium bangladeshense sp. nov. and Rhizobium binae sp. nov. from lentil (Lens culinaris) nodules

Rhizobial strains isolated from effective root nodules of field-grown lentil (Lens culinaris) from different parts of Bangladesh were previously analysed using sequences of the 16S rRNA gene, three housekeeping genes (recA, atpD and glnll) and three nodulation genes (nodA, nodC and nodD), DNA fingerprinting and phenotypic characterization. Analysis of housekeeping gene sequences and DNA fingerprints indicated that the strains belonged to three novel clades in the genus Rhizobium. In present study, a representative strain from each clade was further characterized by determination of cellular fatty acid compositions, carbon substrate utilization patterns and DNA DNA hybridization and average nucleotide identity (ANI) analyses from whole-genome sequences. DNA DNA hybridization showed 50-62 % relatedness to their closest relatives (the type strains of Rhizobium etli and Rhizobium phaseoh) and 50-60 % relatedness to each other. These results were further supported by ANI values, based on genome sequencing, which were 87-92 % with their close relatives and 88-89 % with each other. On the basis of these results, three novel species, Rhizobium lentis sp. nov. (type strain BLR27(T)=LMG 28441(T)=DSM 29286(T)), Rhizobium bangladeshense sp. nov. (type strain BLR175(T)=LMG 28442(T)=DSM 29287(T)) and Rhizobium binae sp. nov. (type strain BLR195(T)=LMG 28443(T)=DSM 29288(T)), are proposed. These species share common nodulation genes (nodA, nodC and nodD) that are similar to those of the symbiovar viciae